Vol 52, Issue 3 – Brazilian Journal of Pathology and Laboratory Medicine

Evaluation of TP53 Gene Expression in Patients with Childhood Cancer in Northeast Santa Catarina, Brazil

Platelet interference in automated reticulocyte counting

Article Type, Editorial, Issue, Vol 52, Issue 3 / cadmin

Thales Gouveia LimeiraJ. Bras. Patol. Med. Lab. 2016;52(3):136DOI: 10.5935/1676-2444.20160027 Retired professor from Universidade Federal do Espírito Santo (Ufes), Espírito Santo, Brazil; Faculdade Multivix, Espírito Santo, Brazil Medicine has gone through an admirable improvement due to the arsenal of resources offered by scientific advancement, what allows for prolongation of life and the enhancement of its quality. […]

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Performance of anti-AF antibodies to distinguish secondary Sjögren's syndrome in RA patients A) ROC of anti-AF IgA with area = 0.502 (95% CI = 0.38-0.62); p = 0.96; B) ROC of anti- AF IgG with area = 0.52 (95% IC = 0.4-0.64); p = 0.67. AF: alpha-fodrin; RA: rheumatoid arthritis; ROC: receiver operating characteristic; IgA: immunoglobulin class A; CI: confidence interval; IgG: immunoglobulin class G.

Anti-alpha-fodrin antibodies in patients with Sjögren’s syndrome secondary to rheumatoid arthritis

Article Type, Issue, Laboratory Medicine, Vol 52, Issue 3 / cadmin

Renato Nisihara; Thelma L. Skare; Elisa Cenci; Denise Gabardo; Flavia Nass; Shirley R. R. UtiyamaJ. Bras. Patol. Med. Lab. 2016;52(3):137-141DOI: 0.5935/1676-2444.20160023 ABSTRACT INTRODUCTION: The Sjögren’s syndrome (SS) is an autoimmune disease characterized by lymphocytic infiltration. The currently most researched antibodies for its diagnosis are anti-La and anti-Ro, which, however, have low specificity in the case of

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Strategy of analysis for reticulocyte count by FC/TO and CD61PE A) selection of the population of interest, R1, based on morphometric aspects for the construction of graph B (FL1 × anti-CD61PE/FL2); B) identification of the homogeneous population, R2, and possible interferents - R3 and R4; C) quantification of the percentage of positive cells in the upper right quadrant (stained with TO) present in the R2 region - graph drawn with the combination of gates in R1 and R2 regions. FC/TO: method of reticulocyte count by flow cytometry, using thiazole orange; SSC: side scatter; FSC: forward scatter.

Evaluation of the interference of platelets in reticulocyte counting by flow cytometry using thiazole orange

Article Type, Issue, Laboratory Medicine, Vol 52, Issue 3 / cadmin

Karina Augusta Viana; Olindo A. Martins Filho; Luci Maria S. Dusse; Lis R. V. Antonelli; Danielle M. V. Avelar; Renato S. Avelar; Maria das Graças CarvalhoJ. Bras. Patol. Med. Lab. 2016;52(3):142-148DOI: 10.5935/1676-2444.20160025 ABSTRACT INTRODUCTION: The reticulocyte count by flow cytometry (FC) – an automated counting method – can present errors due to the presence of interfering

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Flow of test conduction at the core laboratory of HC/UFMG HC/UFMG: Hospital das Clínicas da Universidade Federal de Minas Gerais; LIS: laboratory information system.

Implementation of criteria for automatic release of clinical chemistry test results in a laboratory at an academic public hospital

Article Type, Issue, Laboratory Medicine, Vol 52, Issue 3 / cadmin

Myriam S. Feitosa; Daniel Henrique Bücker; Silvana Maria E. Santos; Leonardo S. VasconcellosJ. Bras. Patol. Med. Lab. 2016;52(3):149-156DOI: 10.5935/1676-2444.20160026 ABSTRACT INTRODUCTION: Autoverification is the release of laboratory test results from clinical instruments to hospital interface, or to patients’ records, with no human intervention. Verification rules are inserted in the middleware and/or in the laboratory information system

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Traceability of the analytical systems reported by the laboratories participating in the proficiency testing program of ControlLab, for determinations of glucose, urea, creatinine, uric acid, and cholesterol, during 2012 and 2013 CRM: certified reference material; RMet: reference method; N-Info: no information on the use of CRM or RMet.

Metrological traceability in clinical laboratory

Article Type, Issue, Laboratory Medicine, Vol 52, Issue 3 / cadmin

Myriam S. Feitosa; Daniel Henrique Bücker; Silvana Maria E. Santos; Leonardo S. VasconcellosJ. Bras. Patol. Med. Lab. 2016;52(3):149-156Maria Cristina F. Pessoa; Orlando C. Ferreira Jr.J. Bras. Patol. Med. Lab. 2016;52(3):157-164DOI: 10.5935/1676-2444.20160028 ABSTRACT INTRODUCTION: Metrological traceability is a little-known and little-discussed metrology theme in clinical laboratories, although it is the basis for comparable results. It is associated

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Frequency of microorganisms isolated from the analyzed blood cultures

Clindamycin microbial resistance in clinical isolates of Staphylococcus sp. derived from blood cultures of hospitalized patients

Article Type, Issue, Laboratory Medicine, Vol 52, Issue 3 / cadmin

Amanda Cristina O. Silva; Ruan Carlos G. Silva; Sibele R. OliveiraJ. Bras. Patol. Med. Lab. 2016;52(3):165-170DOI: 10.5935/1676-2444.20160024 ABSTRACT INTRODUCTION: Infections caused by methicillin-resistant Staphylococcus aureus (MRSA) strains have become common in hospitals, and this resistance has limited patients’ treatment with beta-lactam antibiotics. Clindamycin is an important therapeutic agent for MRSA infections; however, inducible macrolide-lincosamide-streptogramin B (MLSB) resistance (iMLSB)

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Kinetics of the expressions of radiation-induced plasma proteins of the cardiac territory in electrophoresis

Article Type, Issue, Laboratory Medicine, Vol 52, Issue 3 / cadmin

Celso V. Lima; Tarcisio P. R. CamposJ. Bras. Patol. Med. Lab. 2016;52(3):171-177DOI: 10.5935/1676-2444.20160029 ABSTRACT INTRODUCTION: Radiation induces acute and late alterations in blood proteins.OBJECTIVE: The present study aims at analyzing in time kinetics the electrophoretic profiles of expression bands of blood protein, in the molecular weight range greater than and equal to that of albumin, modulated by

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Tests most frequently ordered at UBSs UBS: basic health units; TSH: thyroid-stimulating hormone; HDL-cholesterol: high density lipoprotein cholesterol.

Evaluation of the use of laboratory tests in primary care

Article Type, Issue, Laboratory Medicine, Vol 52, Issue 3 / cadmin

Adriana P. Valle; Paulo José F. Villas Boas; Wanice W. Bicudo; Alessandro F. JacintoJ. Bras. Patol. Med. Lab. 2016;52(3):178-181DOI: 10.5935/1676-2444.20160030 ABSTRACT INTRODUCTION: The evaluation of the use of laboratory tests in primary care can improve the quality of care provided to the population.OBJECTIVE: To evaluate the laboratory tests in primary health care.METHODS: Tutorial groups were set to evaluate

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A peripheral blood smear (100×): Leishman's stain indicating leukoerythroblastic picture in a case of BMN. Black arrows show nucleated erythrocyte precursors, with red arrows indicating granulocytes BMN: bone marrow necrosis.

Bone marrow necrosis: literature review

Article Type, Issue, Pathology, Vol 52, Issue 3 / cadmin

Adriana P. Valle; Paulo José F. Villas Boas; Wanice W. Bicudo; Alessandro F. JacintoJ. Bras. Patol. Med. Lab. 2016;52(3):178-181Tamara C. S. Cabral; Carolina M. Fernandes; Luis Alberto C. Lage; Maria Claudia Zerbini; Juliana PereiraJ. Bras. Patol. Med. Lab. 2016;52(3):182-188DOI: 10.5935/1676-2444.20160031 ABSTRACT INTRODUCTION: Bone marrow necrosis (BMN) is a rare pathologic entity that is commonly undiagnosed, and

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Submucous nodule on the left lateral border of the tongue

Intraoral spindle-cell lipoma with chondroid differentiation: importance in the diagnosis of oral lesions presenting chondroid tissue

Article Type, Issue, Pathology, Vol 52, Issue 3 / cadmin

Ademar Takahama Junior; Michele F. Brantes; Jorge E. Leon; Adriele F. Gouvêa; Oslei P. AlmeidaJ. Bras. Patol. Med. Lab. 2016;52(3):189-193DOI: 10.5935/1676-2444.20160032 ABSTRACT Lipomas are benign neoplasms of adipose tissue presenting several histologic variants, which can be rarely found in the oral cavity. We present a case of a 62-year-old woman with a submucous nodule located

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