Brazilian Journal of Pathology and Laboratory Medicine

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Article Type

Underreporting of scientific knowledge: a theme for reflection

Anti-alpha-fodrin antibodies in patients with Sjögren’s syndrome secondary to rheumatoid arthritis

Anticorpos antialfafodrina em pacientes com síndrome de Sjögren’ secundária a artrite reumatoide Renato Nisihara1, 2; Thelma L. Skare1; Elisa Cenci1; Denise Gabardo1; Flavia Nass3; Shirley R. R. Utiyama3 1. Faculdade Evangélica do Paraná (Fepar), Paraná, Brazil2. Universidade Positivo (UP), Paraná, Brazil3. Universidade Federal do Paraná (UFPR), Paraná, Brazil DOI: 0.5935/1676-2444.20160023 Corresponding author Renato NisiharaRua João […]

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Strategy of analysis for reticulocyte count by FC/TO and CD61PE A) selection of the population of interest, R1, based on morphometric aspects for the construction of graph B (FL1 × anti-CD61PE/FL2); B) identification of the homogeneous population, R2, and possible interferents - R3 and R4; C) quantification of the percentage of positive cells in the upper right quadrant (stained with TO) present in the R2 region - graph drawn with the combination of gates in R1 and R2 regions. FC/TO: method of reticulocyte count by flow cytometry, using thiazole orange; SSC: side scatter; FSC: forward scatter.

Evaluation of the interference of platelets in reticulocyte counting by flow cytometry using thiazole orange

Karina Augusta Viana; Olindo A. Martins Filho; Luci Maria S. Dusse; Lis R. V. Antonelli; Danielle M. V. Avelar; Renato S. Avelar; Maria das Graças CarvalhoJ. Bras. Patol. Med. Lab. 2016;52(3):142-148DOI: 10.5935/1676-2444.20160025 ABSTRACT INTRODUCTION: The reticulocyte count by flow cytometry (FC) – an automated counting method – can present errors due to the presence of interfering

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Underreporting of scientific knowledge: a theme for reflection

Evaluation of the interference of platelets in reticulocyte counting by flow cytometry using thiazole orange

Avaliação da interferência de plaquetas na contagem de reticulócitos por citometria de fluxo, utilizando laranja de tiazol Karina Augusta Viana1; Olindo A. Martins Filho2; Luci Maria S. Dusse1; Lis R. V. Antonelli2; Danielle M. V. Avelar3; Renato S. Avelar3; Maria das Graças Carvalho1 1. Universidade Federal de Minas Gerais (UFMG), Minas Gerais, Brazil2. Centro de

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Underreporting of scientific knowledge: a theme for reflection

Implementation of criteria for automatic release of clinical chemistry test results in a laboratory at an academic public hospital

Implantação de critérios de liberação automática de resultados de bioquímica em um laboratório de hospital público universitário Myriam S. Feitosa1; Daniel Henrique Bücker1; Silvana Maria E. Santos2; Leonardo S. Vasconcellos2 1. Hospital das Clínicas da Universidade Federal de Minas Gerais (HC/UFMG), Minas Gerais, Brazil2. Falcudade de Medicina da UFMG, Minas Gerais, Brazil DOI: 10.5935/1676-2444.20160026 Corresponding

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Flow of test conduction at the core laboratory of HC/UFMG HC/UFMG: Hospital das Clínicas da Universidade Federal de Minas Gerais; LIS: laboratory information system.

Implementation of criteria for automatic release of clinical chemistry test results in a laboratory at an academic public hospital

Myriam S. Feitosa; Daniel Henrique Bücker; Silvana Maria E. Santos; Leonardo S. VasconcellosJ. Bras. Patol. Med. Lab. 2016;52(3):149-156DOI: 10.5935/1676-2444.20160026 ABSTRACT INTRODUCTION: Autoverification is the release of laboratory test results from clinical instruments to hospital interface, or to patients’ records, with no human intervention. Verification rules are inserted in the middleware and/or in the laboratory information system

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Underreporting of scientific knowledge: a theme for reflection

Metrological traceability in clinical laboratory

Rastreabilidade metrológica no laboratório clínico Maria Cristina F. Pessoa1; Orlando C. Ferreira Jr.2 1. Instituto Nacional de Metrologia, Qualidade e Tecnologia (Inmetro), Rio de Janeiro, Brazil2. Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil DOI: 10.5935/1676-2444.20160028 Corresponding author Maria Cristina Ferreira PessoaRua Prefeito João Felipe, 537, apto 103; Santa TeresaCEP: 20251-140; Rio

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Traceability of the analytical systems reported by the laboratories participating in the proficiency testing program of ControlLab, for determinations of glucose, urea, creatinine, uric acid, and cholesterol, during 2012 and 2013 CRM: certified reference material; RMet: reference method; N-Info: no information on the use of CRM or RMet.

Metrological traceability in clinical laboratory

Myriam S. Feitosa; Daniel Henrique Bücker; Silvana Maria E. Santos; Leonardo S. VasconcellosJ. Bras. Patol. Med. Lab. 2016;52(3):149-156Maria Cristina F. Pessoa; Orlando C. Ferreira Jr.J. Bras. Patol. Med. Lab. 2016;52(3):157-164DOI: 10.5935/1676-2444.20160028 ABSTRACT INTRODUCTION: Metrological traceability is a little-known and little-discussed metrology theme in clinical laboratories, although it is the basis for comparable results. It is associated

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Underreporting of scientific knowledge: a theme for reflection

Clindamycin microbial resistance in clinical isolates of Staphylococcus sp. derived from blood cultures of hospitalized patients

Resistência microbiana à clindamicina em isolados clínicos de Staphylococcus sp. provenientes de hemoculturas de pacientes hospitalizados Amanda Cristina O. Silva; Ruan Carlos G. Silva; Sibele R. Oliveira Associação Caruaruense de Ensino Superior e Técnico (Asces), Pernambuco, Brazil DOI: 10.5935/1676-2444.20160024 Corresponding author Amanda Cristina de Oliveira SilvaRua Paraná, 396; Cruz AltaCEP: 55195-003; Santa Cruz do Capibaribe-PE, BrasilPhone: +55

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Frequency of microorganisms isolated from the analyzed blood cultures

Clindamycin microbial resistance in clinical isolates of Staphylococcus sp. derived from blood cultures of hospitalized patients

Amanda Cristina O. Silva; Ruan Carlos G. Silva; Sibele R. OliveiraJ. Bras. Patol. Med. Lab. 2016;52(3):165-170DOI: 10.5935/1676-2444.20160024 ABSTRACT INTRODUCTION: Infections caused by methicillin-resistant Staphylococcus aureus (MRSA) strains have become common in hospitals, and this resistance has limited patients’ treatment with beta-lactam antibiotics. Clindamycin is an important therapeutic agent for MRSA infections; however, inducible macrolide-lincosamide-streptogramin B (MLSB) resistance (iMLSB)

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Underreporting of scientific knowledge: a theme for reflection

Kinetics of the expressions of radiation-induced plasma proteins of the cardiac territory in electrophoresis

Cinética das expressões de proteínas plasmáticas radioinduzidas do território cardíaco em eletroforese Celso V. Lima; Tarcisio P. R. Campos Universidade Federal de Minas Gerais (UFMG), Minas Gerais, Brazil DOI: 10.5935/1676-2444.20160029 Corresponding author Tarcisio Passos Ribeiro de CamposDepto. Engenharia Nuclear; Escola de EngenhariaAv. Antonio Carlos, 6.627, EE, Bloco 4, Sala 2.285CEP: 31270-901; Belo Horizonte-MG, Brasile-mail: tprcampos@pq.cnpq.br First

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