Mariana Alves Rocha
Department of Laboratory Medicine, Universitas Sebelas Maret, Surakarta, Indonesia
Laila Nur Syafitri
Department of Laboratory Medicine, Universitas Sebelas Maret, Surakarta, Indonesia
Bambang Wicaksono Hartono
Department of Laboratory Medicine, Universitas Sebelas Maret, Surakarta, Indonesia
ABSTRACT
Contemporary flow cytometric analysis is a dependable and effective method for examining people with haematological malignancies. In the majority of cases with leukaemia and lymphoma involving the blood and marrow, it is the most significant single inquiry. When cancer cells are present in intricate mixes of normal cells, as is often the case in the bone marrow and lymph nodes, flow cytometry is very useful in detecting them. The sensitivity of detection has risen due to advancements in reagents and equipment, and flow cytometry is now used to monitor patients following therapy in cases where extremely low amounts of residual illness may exist. This enables more accurate therapy administration and treatment result prediction. The quality of the sample is the most significant issue that might restrict the use of flow cytometry. Thus, it is essential to place samples in the appropriate bottles and get them to the lab on time. Using a laser beam in a fluid stream, single cells may have several phenotypic features measured and examined using a laser-based analytical method called flow cytometry. Cell components such as surface proteins are labelled throughout the experiment using fluorescent tags that are often affixed to antibodies. The detector located close to the laser beam detects the wavelengths of light that the fluorescently tagged cells release as they move through the beam. It is possible to investigate the identification of individual cells using their fluorescence and light-scattering characteristics.
Keywords: Flow Cytometry (FC), Diagnosis (DD), Hematologic Disease (HD), Brazil (B).
