Mariana Costa Lima
Department of Clinical Pathology, Universidade de São Paulo (USP), São Paulo, Brazil
Camila Nunes Ferreira
Laboratory of Nanomedicine, Universidade Estadual de Campinas (UNICAMP), Campinas, Brazil
ABSTRACT
The benefits of these molecular techniques for leptospirosis diagnosis are considered, as is Colombia's response to the SARS-CoV-2 pandemic, which made it possible to develop and provide 227 real-time PCR test-capable laboratories in 28 of the 32 departments of the country. It is suggested that a molecular test be standardized to enable the precise diagnosis of serovars and species of Leptospira nationwide. By encouraging the diagnosis of leptospirosis during its acute phase, this approach may help lower the underreporting of leptospirosis-related morbidity and death in rural area. Additionally, it would support healthcare professionals in managing the condition, minimizing antibiotic usage, reducing patient problems and hospital admissions, and contributing to a more accurate diagnosis of the disease. It would also help minimize the costs and time spent on laboratory diagnostic testing. The important implication of a rapid PCR Test for the detection of Leptospirosis is that it can be useful for the evaluation of vaccine efficiency in a particular population. As described earlier, the vaccine is the best preventive method against disease, but some factors can reduce the efficiency of the vaccine in any particular population in rural areas. However, PCR-based Tests can effectively evaluate vaccine efficiency in any targeted population. This can also be used to evaluate vaccine efficiency in the human population and livestock.
Keywords: Rapid PCR-Based Test (PCR), Detection (DD), Leptospirosis (LL), Rural Area (RA).
