Salam Maseer Juma’a
Department of Biology, College of Education for Pure Science, University of Diyala, Diyala, Iraq
Thekra Atta Ibrahim
Department of Biology, College of Education for Pure Science, University of Diyala, Diyala, Iraq
ABSTRACT
Background: B-complex vitamins, particularly in the form of Neurobion (a combination of vitamins B1, B6, and B12), are widely used for their neuroprotective and analgesic properties. However, their potential impact on renal function remains inadequately studied. This study aimed to evaluate the histopathological effects of Neurobion administration on kidney structure in Swiss albino mice (Mus musculus). Methods: A total of 10 Swiss albino mice were divided into two groups (n = 5 per group). The control group received no treatment, while the experimental group was intraperitoneally injected with Neurobion (0.2 mg/kg) daily for 30 days. The animals were housed in separate cages with continuous monitoring. At the end of the study, the mice were euthanized, and kidney tissues were extracted for histopathological examination. Results: Behavioral and physiological changes were observed in the Neurobion-treated group, including reduced water intake, decreased appetite, and weight loss. Histopathological analysis revealed significant renal alterations, including glomerular atrophy, Bowman’s space dilation, and tubular necrosis. Degenerative changes were observed in renal tubular epithelial cells, accompanied by swelling, nuclear hypertrophy, and inflammatory cell infiltration. Vascular congestion and thrombosis were also evident, along with thickening of blood vessel walls. Additionally, Periodic Acid-Schiff (PAS) staining demonstrated glycogen accumulation in tubular cell basement membranes and Bowman’s capsule, suggesting metabolic alterations in renal tissue. Conclusion: The findings suggest that prolonged administration of Neurobion (B1, B6, B12) induces histopathological changes in renal tissue, potentially affecting kidney function. These alterations, including tubular degeneration, glomerular damage, and vascular changes, indicate possible nephrotoxic effects. Further research is necessary to assess the clinical implications and underlying mechanisms of B-vitamin supplementation on renal health.
Keywords: Neurobion, Albino Mice, Kidneys, Bowman’s Space.
