Hasan Hussein Idan
Dept. of Biochemistry, College of Medicine, University of Baghdad, Iraq
Halla Ghazi Mohamoud
Dept. of Biochemistry, College of Medicine, University of Baghdad, Iraq
ABSTRACT
Background: Type 2 diabetes mellitus (T2DM) is characterized by chronic oxidative stress and inflammation, which contribute to disease progression and the development of complications. Myeloperoxidase (MPO) and malondialdehyde (MDA) are biomarkers associated with oxidative stress and inflammatory processes, making them potential indicators for assessing T2DM-related complications. Objective: This study aimed to evaluate the clinical significance of serum MPO and MDA levels as biomarkers for complications in Iraqi patients with T2DM. Methods: A total of 130 participants were enrolled, including 70 patients with T2DM and 60 healthy controls. Serum MPO and MDA levels were measured using enzyme-linked immunosorbent assay (ELISA), while glycated hemoglobin (HbA1c) was analyzed using an automated biochemical analyzer. Statistical analysis was performed using Pearson correlation and Student’s t-test. The diagnostic accuracy of MPO and MDA was assessed through receiver operating characteristic (ROC) curve analysis. Results: Serum levels of MPO and MDA were significantly elevated in T2DM patients compared to healthy controls (p < 0.01). Pearson correlation analysis demonstrated a strong positive correlation between MPO, MDA, and HbA1c levels, indicating an association with glycemic control and disease severity. ROC curve analysis revealed high area under the curve (AUC) values for both MPO (0.98) and MDA (0.96), suggesting their potential utility as predictive biomarkers for T2DM complications. Conclusion: The findings suggest that elevated MPO and MDA levels are strongly associated with oxidative stress and metabolic dysfunction in T2DM patients. Both biomarkers show promise as potential indicators for identifying individuals at high risk of developing diabetes-related complications. Further studies are recommended to explore their clinical applicability in disease monitoring and management.
Keywords: Myeloperoxidase, Malondialdehyde, Type 2 Diabetes Mellitus, Diabetes Mellitus, Complications of Diabetes.
