Zahraa F. Shaker
Baghdad, Iraq
Manal M. Kadhim
Professor of Clinical Immunology, Department of Medical Microbiology, College of Medicine, University of Al-Qadisiyah, Diwaniya, Iraq
ABSTRACT
Background: Recurrent pregnancy loss (RPL), defined as two or more consecutive failed clinical pregnancies confirmed by ultrasonography or histopathology, remains a significant clinical challenge. MicroRNAs (miRNAs) are critical regulators of gene expression during the preimplantation phase and may influence pregnancy outcomes. Purpose: This study aimed to explore the association between genetic polymorphisms in miRNAs (miR-27a and miR-938G>A) and recurrent pregnancy loss in Iraqi females. Methods: The study involved forty women diagnosed with idiopathic RPL at the departments of infertility, gynecology, and obstetrics in Baghdad Hospital City from February to October 2022, along with a control group of 40 healthy individuals. Genotyping of miR-27a and miR-938G>A was performed using polymerase chain reaction (PCR) on samples of whole blood. Results: Our findings revealed a significant correlation between the miR-27a GG and AG genotypes and a reduced risk of RPL. Conversely, the miR-938G>A polymorphism was strongly associated with an increased risk of RPL (odds ratio for G/A vs. GG = 36; 95% confidence interval: 9.89 - 130.9). Individuals possessing both miR-27a A/G and miR-938 G/A genotypes were found to have a higher likelihood of experiencing recurrent pregnancy losses. Conclusion: The study confirms that specific single nucleotide polymorphisms (SNPs) in miR-27a and miR-938 are associated with the risk of RPL. These miRNAs could serve as valuable biomarkers for predicting RPL, thereby aiding in the risk assessment and management of potentially affected women. Further research is encouraged to validate these findings and explore their potential diagnostic utility in clinical settings.
Keywords: Recurrent pregnancy loss, miRNA-27a A/G, and miRNA-938 G/A.
