Fatima M. Bader
Department of Biology- Qurna Education Collage \ University of Basrah, Iraq
Kawakib I. Al-zubaidy
Department of Biology- Qurna Education Collage \ University of Basrah, Iraq
ABSTRACT
Background: Aloe Vera has been traditionally used for its therapeutic properties in treating various skin conditions. This study investigates the histopathological effects and antibacterial properties of alcoholic Aloe Vera gel extract on wounds infected with Staphylococcus aureus in a murine model. Methods: Thirty-two mice were divided into four groups: a treatment group infected with S. aureus and treated with alcoholic Aloe Vera gel extract, a positive control group infected with S. aureus only, a negative control group with only the wound, and a healthy control group. The effectiveness of Aloe Vera was assessed through histopathological examination and measurement of wound diameters at various stages of the healing process. Results: The treated group showed significant inhibition of S. aureus and accelerated wound healing compared to controls. At day four, wound diameters were significantly smaller in the treated group (9.000 mm) compared to the positive (12.500 mm) and negative controls (11.000 mm). This trend continued, with the treated group showing a wound diameter of 3.500 mm at day twenty-one, compared to 5.500 mm and 4.500 mm in the positive and negative controls, respectively. Histopathological analysis revealed enhanced repair processes in the treated group, characterized by the early appearance of granulation tissue and a more rapid resolution of inflammation. The positive control sections displayed persistent inflammatory cells and slower repair kinetics, while the negative control showed delayed formation of skin layers. Conclusion: The alcoholic extract of Aloe Vera gel significantly modulates the pathophysiological response to S. aureus infection in wounds, demonstrating potent antibacterial and wound-healing properties. These findings suggest that Aloe Vera could be a valuable agent in enhancing skin repair mechanisms, with implications for both clinical and pathophysiological applications in wound management.
Keywords: Aloe Vera gel, Staphylococcus aureus, wound healing, histopathology.
